Group A01:Measurement and control of cell characteristics

Through planned research, we intend to establish and systematize methodologies for the measurement of cellular characteristics using high-speed micro-nano robot technology, and for ultra high-speed separation of active and uncommon cells that are useful in the construction of three-dimensional (3D) cellular systems. We will establish techniques to grow cells extracted from living organisms and perform ultra high-speed separation of active cells that are useful in tissue formation. We aim to understand the multifaceted properties of cells and, focusing on those properties, develop the world’s fastest measurement and separation techniques with the application of micro-nano robotics. The findings will then be applied to medicine.

Research item Research Leader Affiliation Area of expertise Planned Research
A01-1 Fumihito Arai
Detail
Professor, Nagoya University
A01 Leader
Micro-Nano Mechatronics Measurements of cells and monitoring of autonomous induction with ultra high-speed manipulations
A01-2 Makoto Kaneko
Detail
Professor, Osaka University Mechatronics Engineering Ultra-High Speed Measurement of Cell Properties
A01-3 Hiromitsu Nakauchi
Detail
Tokyo University
Professor,Institute of Medical Sciences
Stem Cell Biology Developing system capable of a closed, high-speed cell analysis and separation which targets cells with very limited numbers in vivo
A01 Takaharu Okajima
Detail
Hokkaido University
Associate Professor
Biophysics High-speed measurement of cell rheology by atomic force microscopy
A01 Idaku Ishii
Detail
Hiroshima University
Professor
Robotics

Vision-based Sensing
Real-Time Micro PIV System for Cell-Oriented Active Sensing
A01 Tomoyuki Yasukawa
Detail
University of Hyogo
Associate Professor
Analytical chemistry

Bioelectrochemistry
Rapid patterning with cells and high-throughput discrimination >of cellular differentiation

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Group A02:Construction of 3D cellular systems

Research Group A02 (Assembly Group) studies 3D cellular system architecture. Using high-speed micro-nano robot technology, the Assembly Group forms linear, 2D, and 3D configurations of cells and applies layering, rolling, and folding techniques with the aim of establishing and systematizing methodologies to assemble and construct diverse 3D cellular systems. Research topics in the Assembly Group are construction of 3D cellular systems using ultra high-speed fine manipulation technology (Tatsuo Arai), ultra high-speed assembly of 3D cellular systems based on nano-scale ultra high-speed cell sorting and manipulation (Toshio Fukuda), high-speed cell assembly that takes advantage of fluidics (Minoru Seki), and construction of 3D tissue from cells using MEMS (Shoji Takeuchi). Research is conducted while maintaining organic cooperation with Groups A01 and A03.

Research item Research Leader Affiliation Area of expertise Planned Research
A02-1 Tatsuo Arai
Detail
Professor, Osaka University
A02 Leader
Robotics Construction of 3D cellular system using high-speed micro manipulation
A02-2 Toshio Fukuda
Detail
Professor, Nagoya University Robotics

Micro-Nano Systems Engineering
Ultra high speed assembly of 3D cell systems based on nano-scale cell selection/operation
A02-3 Minoru Seki
Detail
Professor, Chiba University Biochemical engineering High speed cell assembly using fluidics
A02-4 Shoji Takeuchi
Detail
Associate Professor, Tokyo University Microfluidic devices Construction of 3D cellular structures using MEMS
A02 Hirofumi Matsui
Detail
University of Tsukuba
Associate Professor
- Four-dimensional analysis for a tumor invasion
A02 Junji Fukuda
Detail
University of Tsukuba
Assistant Professor
Biomedical Engineering Rapid molding of vascular-like structure for bone tissue engineering
A02 Hitoshi Yoshikawa
Detail
Saitama University
Assistant Professor
Biophysics, Optics Development of dynamically tunable biointerfaces and its application for 3D cellular systems.
A02 Shinji Sugiura
Detail
National Institute of Advanced Industrial Science and Technology
Senior Research Scientist
Biochemical Engineering
Microprocess Engineering
Photochemical fabrication of three-dimensional tissue construct

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Group A03:Analysis and evaluation of 3D cellular systems

This group quantitatively measures the functions of reconstituted 3D tissues made using technologies developed by Groups A01 and A02, after these tissues have been transplanted to culture systems or experimental animals. The group also attempts to shed light on growth, differentiation-inducing, and morphogenesis controls and transplantation responses, elucidate functions and compare and verify in vitro, and apply its findings to regenerative medicine. Specifically, targeting soft tissue such as the liver and hard tissue such as bone, the group produces co-cultured cell sheets from patterned co-cultured cell surfaces and constructs 3D tissue structures with layering of these sheets. The group also quantitatively evaluates similarity and functional expression of reconstituted 3D tissue and physiological tissue using confocal laser scanning microscopy, live cell imaging systems, and immunohistological investigations; and clarifies the reconstitution maturation process and vascular introduction process in vivo by transplanting into immunodeficient mice or other systems.

The group also measures physical and chemical processes in liver and bone formation environments, which, to date, has been difficult, and intends to elucidate the expression mechanism of complex physiological functions that are first expressed when cells act together as a group. The group aims to develop these technologies and promote their application in medicine.

Research item Research Leader Affiliation Area of expertise Planned Resarch
A03-1 Masayuki Yamato
Detail
Professor, Tokyo Women's Medical University
A03 Leader
Regenerative,
Stem Cell Biology
Reconstruction and functional elucidation of complex tissue of the liver and other organs
A03-2 Osamu Suzuki
Detail
Professor, Tohoku University Calcification and Bone regeneration Clarification of bone mineralization process and hard tissue construction
A03 Takeomi Mizutani
Detail
Hokkaido University
Assistant Professor
Biophysics Tempo-spatial dynamics of cellular mechanics during 3D morphogenesis
A03 Taisuke Masuda
Detail
Nagoya University
Assistant Professor
Bio MEMS Bionic simulator in Organ-Explant-Chip
A03 Takanori Kihara
Detail
Osaka University
Assistant Professor
Cell and tissue engineering Cell dynamics analysis and modeling of various calcified tissues
A03 Takuya Matsumoto
Okayama University
Professor
- Reproduced microenvironment for In vitro gland tissue synthesis and understanding the related mechanics
A03 Takanori Takebe
Yokohama City University
Assistant Professor
- Visualization of human organ development through the continuous live imaging